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Updated: Jun 11, 2026

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
Published on: April 23, 2019
Discrimination of saturated aldehydes by the rat I7 olfactory receptor
Michael D Kurland1, Michael B Newcomer, Zita Peterlin
1Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.
Abstract:
The discrimination of n-alkyl-saturated aldehydes during the early stage of odorant recognition by the rat I7 olfactory receptor (OR-I7) is investigated. The concentrations of odorants necessary for 50% activation (or inhibition) of the OR-I7 are measured by calcium imaging recordings of dissociated rat olfactory sensory neurons, expressing the recombinant OR-I7 from an adenoviral vector. These are correlated with the corresponding binding free energies computed for a homology structural model of the OR-I7 built from the crystal structure of bovine visual rhodopsin at 2.2 A resolution.
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