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Glass-bead column separation of motile and nonmotile human spermatozoa
N Lechtzin1, W Garside, S Heyner
1Department of Biology, Temple University, Philadelphia, Pennsylvania 19122.
Journal of in Vitro Fertilization and Embryo Transfer : IVF
|April 1, 1991
Abstract:
Glass-bead columns were tested for their efficiency in concentrating motile human spermatozoa from frozen semen samples. The data show that glass-bead filtration concentrates the motile gametes in each sample and is significantly more efficient than the swim-up method for obtaining populations of motile spermatozoa. The data suggest that this method can be applied clinically to obtain motile spermatozoa from poor-quality semen for use in in vitro fertilization.