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Updated: Jun 11, 2026
A Multi-detection Assay for Malaria Transmitting Mosquitoes
Published on: February 28, 2015
Laboratory and field testing of bednet traps for mosquito (Diptera: Culicidae) sampling in West Java, Indonesia
Craig A Stoops1, Yoyo R Gionar, Saptoro Rusmiarto
1Navy Medical Research Unit 2, U. S. Embassy Jakarta, Unit 8132 NAMRU 2, FPO AP 96520-8132, Indonesia.
Abstract:
Surveillance of medically important mosquitoes is critical to determine the risk of mosquito-borne disease transmission. The purpose of this research was to test self-supporting, exposure-free bednet traps to survey mosquitoes. In the laboratory we tested human-baited and unbaited CDC light trap/cot bednet (CDCBN) combinations against three types of traps: the Mbita Trap (MIBITA), a Tent Trap (TENT), and a modified Townes style Malaise trap (TSM). In the laboratory, 16 runs comparing MBITA, TSM, and TENT to the CDCBN were conducted for a total of 48 runs of the experiment using 13,600 mosquitoes. The TENT trap collected significantly more mosquitoes than the CDCBN. The CDCBN collected significantly more than the MBITA and there was no difference between the TSM and the CDCBN. Two field trials were conducted in Cibuntu, Sukabumi, West Java, Indonesia. The first test compared human-baited and unbaited CDCBN, TENT, and TSM traps during six nights over two consecutive weeks per month from January, 2007 to September, 2007 for a total of 54 trapnights. A total of 8,474 mosquitoes representing 33 species were collected using the six trapping methods. The TENT-baited trap collected significantly more mosquitoes than both the CDCBN and the TSM. The second field trial was a comparison of the baited and unbaited TENT and CDCBN traps and Human Landing Collections (HLCs). The trial was carried out from January, 2008 to May, 2008 for a total of 30 trap nights. A total of 11,923 mosquitoes were collected representing 24 species. Human Landing Collections captured significantly more mosquitoes than either the TENT or the CDCBN. The baited and unbaited TENT collected significantly more mosquitoes than the CDCBN. The TENT trap was found to be an effective, light-weight substitute for the CDC light-trap, bednet combination in the field and should be considered for use in surveys of mosquito-borne diseases such as malaria, arboviruses, and filariasis.
