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Updated: Jun 11, 2026

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
Published on: February 28, 2013
Smoothened antagonists for hair inhibition
Jie Jack Li1, Veerabahu Shanmugasundaram, Satya Reddy
1Department of Chemistry, Michigan Laboratories, Pfizer Global Research & Development, 2800 Plymouth Rd., Ann Arbor, MI 48105, USA. jack.li@bms.com
Abstract:
A series of aminomethylpyrazoles were prepared and evaluated using cell-based Smoothened beta-lactamase reporter assay and Smoothened binding assay. Potent Smoothened antagonists 10k and 10l were found to inhibit hair growth in vivo in the C3H/HeN mouse hair growth model. The more selective compound 10l was tested negative in the 3T3 NRU assay, indicating a low risk for causing photo-irritation and was efficacious using the C3H/HeN mouse hair growth model although it was slightly less efficacious than that of the reference compound eflornithine (7).
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