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DNA Replication

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Eukaryotes

Replication in Eukaryotes

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

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Related Experiment Video

Updated: Jun 11, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

DNA replication: metazoan Sld3 steps forward.

Yu V Fu¹, Johannes C Walter

¹Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA.

Current Biology : CB

|July 13, 2010

Summary

This summary is machine-generated.

In yeast, cyclin-dependent kinases phosphorylate Sld3 to initiate replication. A new study identifies Treslin/Ticrr as the Sld3 ortholog in metazoans, crucial for DNA replication.

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Related Experiment Videos

Last Updated: Jun 11, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Replication initiation is a critical cell cycle process.
Sld3 phosphorylation by cyclin-dependent kinases is vital for replication initiation in yeast.
Metazoans possess three candidate Sld3 homologs.

Discussion:

This study investigates the function of Treslin/Ticrr in metazoan DNA replication.
Treslin/Ticrr is proposed as the functional Sld3 ortholog.
Understanding Sld3 homologs is key to deciphering replication control.

Key Insights:

Treslin/Ticrr is identified as the Sld3 ortholog in metazoans.
This finding clarifies the conservation of replication initiation factors.
The study provides a foundation for further research into Treslin/Ticrr function.

Outlook:

Further studies will explore Treslin/Ticrr's precise role in the replication initiation complex.
Investigating Treslin/Ticrr in various metazoan models will enhance our understanding.
This research opens avenues for exploring therapeutic targets in replication-related diseases.