Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Eukaryotes

Replication in Eukaryotes

Telomeres and Telomerase

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.

Telomeres and Telomerase

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Topoisomerase IIIα resolves inter- and intra-molecular intertwines during DNA replication.

bioRxiv : the preprint server for biology·2026

Same author

Inherited long telomeres induce a genome-wide transcriptional response in budding yeast.

Genetics·2026

Same author

Novel Bacterial Topoisomerase Inhibitors: A New Front in an Old War.

Journal of molecular biology·2026

Same author

Distinct repair outcomes from single and convergent replication fork collapse.

Nature structural & molecular biology·2026

Same author

Strand-independent degradation of uncoupled forks by EXO1 activates ATR and restrains synthesis.

bioRxiv : the preprint server for biology·2026

Same author

Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms.

Nucleic acids research·2025

Search research articles

Related Experiment Video

Updated: Jun 11, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Telomere replication: Mre11 leads the way.

James M Dewar¹, David Lydall

¹Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, Tyne-and-Wear NE4 5PL, UK.

|July 13, 2010

Summary

The Mre11 complex is crucial for recruiting telomerase to leading-strand telomeres in budding yeast. This finding highlights a new role for Mre11 in telomere maintenance.

Area of Science:

Molecular biology
Cellular processes
Genetics

Background:

Telomeres protect chromosome ends from degradation.
Telomerase is essential for telomere maintenance and replication.
The Mre11 complex is known for its role in DNA repair.

Discussion:

Faure et al. investigated the Mre11 complex's function in telomere biology.
The study focused on the differential recruitment of telomerase to leading and lagging strands.
Budding yeast was used as the model organism.

Key Insights:

The Mre11 complex plays a critical role in recruiting telomerase.
This recruitment is specific to leading-strand telomeres.
Lagging-strand telomeres do not show Mre11-dependent telomerase recruitment.

More Related Videos

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

Related Experiment Videos

Last Updated: Jun 11, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

Outlook:

Further research may elucidate the precise mechanism of Mre11-mediated telomerase recruitment.
Understanding this process could have implications for aging and cancer research.
Investigating Mre11's role in other organisms may reveal conserved functions.