Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

DNA methylation reprogramming in marsupial embryos is restricted to the extraembryonic lineage.

Nature communications·2026

Same author

The Impact of Low-Protein Diet on the Molecular and Cellular Development of the Fetal Kidney.

Journal of the American Society of Nephrology : JASN·2026

Same author

Feeder-free yet still naïve: improved method for capturing human pluripotent stem cells.

The EMBO journal·2026

Same author

Post-replicative chromatin accessibility predicts cell fate change.

Stem cell reports·2026

Same author

Morphomechanic tuning of ERK by actin-TFII-IΔ regulates cell identity.

bioRxiv : the preprint server for biology·2026

Same author

Modulation of Nudt21 levels reveals dose-dependent roles of alternative polyadenylation in tissue regeneration.

Nature communications·2026

Same journal

Patient-derived organoids reveal ductal dysfunction and CFTR-modulator responses in chronic pancreatitis.

Cell stem cell·2026

Same journal

Lineage plasticity driven by GATA6 loss fuels colorectal cancer metastasis.

Cell stem cell·2026

Same journal

Quantitative molecular cartography of emergency myelopoiesis reveals conserved modules of hematopoietic activation.

Cell stem cell·2026

Same journal

Paired pre- and post-transplant human immunoprofiling identifies an IFN-γ-JAK1 axis limiting stem-cell-derived RPE engraftment.

Cell stem cell·2026

Same journal

ENPP1 blockade with a humanized monoclonal antibody enhances renal repair after acute kidney injury.

Cell stem cell·2026

Same journal

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 11, 2026

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

When fibroblasts MET iPSCs.

Jose M Polo¹, Konrad Hochedlinger

¹Howard Hughes Medical Institute at Massachusetts General Hospital, Cancer Center, 185 Cambridge Street, Boston, MA 02114, USA.

|July 13, 2010

Summary

This summary is machine-generated.

Induced pluripotent stem cells (iPSCs) arise from a mesenchymal-to-epithelial transition, a process crucial for reprogramming. This finding reveals striking parallels between cellular reprogramming, development, and cancer progression.

More Related Videos

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Published on: January 19, 2020

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

Published on: September 14, 2019

Related Experiment Videos

Last Updated: Jun 11, 2026

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Published on: January 19, 2020

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

Published on: September 14, 2019

Area of Science:

Stem cell biology
Cellular reprogramming
Developmental biology
Cancer biology

Background:

Induced pluripotent stem cells (iPSCs) offer a powerful tool for regenerative medicine and disease modeling.
Understanding the initial events in iPSC derivation is key to improving reprogramming efficiency.

Discussion:

Two independent studies highlight the mesenchymal-to-epithelial transition (MET) as a critical early step in iPSC generation from fibroblasts.
This MET process mirrors events observed during embryonic development and cancer progression.

Key Insights:

The MET is a conserved and fundamental cellular process across different biological contexts.
Fibroblast reprogramming into iPSCs involves a developmental-like transition.

Outlook:

Further research into MET could optimize iPSC generation protocols.
Exploring MET similarities may provide new insights into developmental disorders and cancer therapies.