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Related Experiment Videos

Suspected allergy: a sequential protocol for testing patients' sera.

D R Walkingshaw1, D O Ho-Yen

  • 1Microbiology Dept., Raigmore Hospital, Inverness, Scotland, UK.

Medical Laboratory Sciences
|January 1, 1991
PubMed
Summary
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Elevated total immunoglobulin E (IgE) levels correlate with increased specific allergen reactivity. A new sequential testing protocol for allergy diagnosis optimizes efficiency while ensuring accurate identification of positive RAST results.

Area of Science:

  • Clinical immunology
  • Allergen-specific diagnostics

Background:

  • Total immunoglobulin E (IgE) levels are a common marker in allergy diagnostics.
  • Radio-allergosorbent test (RAST) is used to detect specific IgE antibodies.
  • The relationship between total IgE and specific allergen reactivity requires further clarification for optimized diagnostic strategies.

Purpose of the Study:

  • To investigate the correlation between total IgE concentration and specific allergen reactivity using RAST.
  • To develop and evaluate a sequential diagnostic protocol for suspected allergies.

Main Methods:

  • Analysis of serum samples from 591 patients for total IgE and reactivity to seven specific allergens via RAST.
  • Statistical analysis to determine the relationship between total IgE levels and RAST results.

Related Experiment Videos

  • Development of a sequential testing protocol based on initial total IgE and specific allergen screening.

    • Main Results:

    • A significant positive correlation was observed between increasing total IgE levels and the frequency, number, and grade of positive RAST reactions (P < 0.001).
    • 12% of patients with normal total IgE levels exhibited positive RAST results.
    • The proposed sequential protocol demonstrated reduced unnecessary testing while maintaining the ability to identify positive RAST results.

    Conclusions:

    • Total IgE levels are a valuable indicator of overall allergic sensitization.
    • A sequential diagnostic approach, starting with total IgE and key allergen RAST, is effective in streamlining allergy testing.
    • This protocol enhances diagnostic efficiency for patients with suspected allergies.