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Microelectrode investigation of neuroneal ageing from a single identified neurone
Bhavik Anil Patel1, Martin Arundell, Kim H Parker
1Department of Bioengineering, Imperial College London, London, SW7 2AZ, UK.
Abstract:
Microelectrode amperometry is uniquely suited for characterising the dynamics of neurotransmitter release, as it offers unparalleled spatial and temporal resolution. We have used carbon fibre microelectrodes to study release of the monoamine neurotransmitter serotonin (5-HT) and the gaseous transmitter nitric oxide (NO) in intact central nervous system of the water snail, Lymnaea stagnalis. Analysis of spontaneous vesicular release of 5-HT and depolarisation-induced release of NO reveals significant differences with ageing that may be associated with changes in protein structure and function.
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