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Two hypervariable minisatellite DNA binding proteins.

W P Wahls1, G Swenson, P D Moore

  • 1Department of Genetics, University of Illinois, Chicago 60612.

Nucleic Acids Research
|June 25, 1991
PubMed
Summary
This summary is machine-generated.

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Researchers identified two novel proteins, Msbp-2 and Msbp-3, that bind to hypervariable minisatellite DNA sequences. These proteins may play a role in initiating DNA recombination in human cells.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Hypervariable minisatellite DNA sequences are repetitive DNA elements found in eukaryotes.
  • These sequences are known to stimulate intermolecular homologous recombination in human cells.
  • They are implicated as potential initiation sites for genomic recombination.

Purpose of the Study:

  • To detect and partially purify proteins that bind to hypervariable minisatellite DNA.
  • To investigate the role of these binding proteins in DNA recombination.

Main Methods:

  • Nuclear extracts from human HeLa cells were prepared.
  • Gel filtration chromatography was used for protein purification and mass determination.
  • Detection of DNA-binding proteins was performed.

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Main Results:

  • Two distinct hypervariable minisatellite DNA binding proteins, Msbp-2 and Msbp-3, were identified.
  • Msbp-2 has a subunit size of 77 kDa, and Msbp-3 has a subunit size of 115 kDa.
  • Both proteins eluted from a gel filtration column with a native mass of 200-250 kDa.

Conclusions:

  • Msbp-2 and Msbp-3 are novel proteins that bind to hypervariable minisatellite DNA.
  • These proteins may be involved in the regulation of DNA recombination initiation.