Related Experiment Videos
A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays
J Van Ness1, S Kalbfleisch, C R Petrie
1MicroProbe Corporation, Bothell, WA 98021.
Nucleic Acids Research
|June 25, 1991
Abstract:
A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tailed ODNs are selectively activated using 2,4,6-trichloro-1,3,5-triazine (cyanuric chloride) and then covalently attached to the bead via the triazine moiety. The modified nylon support has a low level of binding of nonspecific nucleic acid and efficiently captures both RNA and DNA targets.