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Updated: Jun 11, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
[Drug-resistance in Mycobacterium tuberculosis in Castilla y León, Spain, 2001-2005: third collaborative study]
Armando Alberte-Castiñeiras1, Angel Campos-Bueno, Luis López-Urrutia
1Microbiología, Hospital Universitario Río Hortega de Valladolid, Valladolid, España. aalberteca@saludcastillayleon.es
Introduction:
During 2001-2005, a regional anti-tuberculosis drug resistance survey was conducted in Castilla y León, Spain, in newly treated HIV negative tuberculosis (TB) patients.
Methods:
A total of 918 Mycobacterium tuberculosis strains were studied (one strain per patient) from six hospitals corresponding to 46.7% of the total population of Castilla y León, using the proportion method on solid medium.
Results:
Primary drug resistance was 4.2% (streptomycin 1.2%, isoniazid 3.2%, rifampin 0.3%, ethambutol 0.1% and pyrazinamide 0.5%). Mono-resistance was observed in 24 (2.6%) and resistance to both isoniazid and rifampin (multi-drug resistance) was detected in one case (0.1%). These results were not statistically significant compared to previous studies in the same Community.
Conclusion:
The incidence of primary drug resistance in the surveyed area was low, including isoniazid, allowing new anti-tuberculosis treatment with the standardised three-drug regimen to be started. Regular surveillance of drug resistance is recommended by the TB control programme in representative patient populations to optimize treatment regimens.
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