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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
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iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
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Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...

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Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics
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Published on: June 17, 2022

Structure-switching biosensors: inspired by Nature.

Alexis Vallée-Bélisle1, Kevin W Plaxco

  • 1Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106, USA.

Current Opinion in Structural Biology
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PubMed
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This summary is machine-generated.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Sensor Technology

Background:

  • Nature utilizes biomolecular switches for real-time chemical sensing.
  • These natural biosensors offer inspiration for artificial sensor design.
  • Adapting biomolecular switches enables advanced chemical detection.

Purpose of the Study:

  • To review the design and application of artificial chemical sensors based on biomolecular switches.
  • To highlight advances in protein and nucleic acid engineering for sensor development.
  • To discuss the integration of optical and electrochemical readouts for complex sample analysis.

Main Methods:

  • Protein and nucleic acid engineering (computational design, directed evolution).
  • Development of structure-switching sensors.
  • Integration of advanced optical (genetically encoded fluorophores) and electrochemical readouts.

Main Results:

  • Enhanced ability to design novel structure-switching sensors.
  • Successful deployment in real-time in vivo imaging.
  • Continuous monitoring of drugs in blood serum demonstrated.

Conclusions:

  • Biomolecular switch-based sensors offer powerful tools for chemical detection.
  • Advances in engineering and readout technologies are driving sensor applications.
  • These artificial sensors mimic natural systems for sophisticated monitoring.