Sexual dimorphism in [3H]histamine binding sites of rat cerebral cortex

[3H]Histamine binding sites, identified on rat brain homogenate membranes, displayed sexual dimorphism with higher density and lower affinity in preparations from adult female compared to the males. The ontogenetic development of [3H]histamine binding sites was studied in male and female brain cortex neural membranes. Histamine binding sites were detectable in newborn-10 day old rats. Density increased with age, reaching adult levels at 37-45 days. No significant differences between sexes were observed in the binding constants until 37 days after birth, when the [3H]histamine binding characteristics began to differentiate according to sex. Sexual dimorphism in brain histamine binding site development appeared to depend on ovarian steroids. Binding patterns in immature female rats which were ovariectomized at 21 days and killed at 37+ were similar to those found in males. On the other hand, when oestradiol replacement was administered for 10 days to ovariectomized rats a total recovery of [3H]histamine binding pattern was obtained, which was comparable to that observed in intact female rats of the same age.