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Updated: Jun 10, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Microarray-based analysis of cell-cycle gene expression during spermatogenesis in the mouse.

Dipanwita Roy Choudhury¹, Chris Small, Yufeng Wang

¹Department of Biology, University of Texas at San Antonio, San Antonio, Texas, USA.

Biology of Reproduction

|July 16, 2010

Summary

This summary is machine-generated.

This study reveals distinct cell-cycle gene networks governing mammalian spermatogenesis. Key gene expression changes occur between mitotic spermatogonia, meiotic spermatocytes, and spermatids, highlighting stage-specific regulation.

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Last Updated: Jun 10, 2026

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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

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Published on: April 10, 2018

Area of Science:

Reproductive biology
Molecular genetics
Cell biology

Background:

Mammalian spermatogenesis involves sequential stages: spermatogonia (mitotic), spermatocytes (meiotic), and spermatids (postreplicative).
Understanding cell-cycle gene regulation is crucial for male fertility and reproductive health.

Purpose of the Study:

To identify cell-cycle gene expression changes across key spermatogenesis stages.
To elucidate distinct regulatory networks governing cell cycle progression in male germ cells.

Main Methods:

Microarray analysis was employed to profile gene expression.
Comparative analysis focused on mitotic spermatogonia, pachytene spermatocytes, and round spermatids.

Main Results:

Expression of 550 cell-cycle-related genes was detected across spermatogenic cells.
Significant transcript level changes were observed between mitotic, meiotic, and postreplicative stages.
221 previously unannotated cell-cycle genes, including 8 testis-specific genes, were identified.

Conclusions:

Distinct cell-cycle gene regulatory networks are associated with each phase of spermatogenesis.
Stage-specific expression of cell-cycle gene family members suggests specialized roles.
The findings expand the known cell-cycle network in spermatogenesis and identify novel testis-specific genes.