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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

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Related Experiment Video

Updated: Sep 17, 2025

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

Inhibiting Aldehyde Dehydrogenase Function Using WIN 18,446 to Synchronize Spermatogenesis.

Shelby L Havel¹, Michael D Griswold²

¹School of Molecular Biosciences, Washington State University, Pullman, WA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 2, 2025

Summary

This summary is machine-generated.

Researchers developed a new method to study sperm development. By blocking a key molecule, they can now analyze specific stages of spermatogenesis with high precision.

Keywords:

Aldehyde dehydrogenases Germ cells Retinoic acid Retinoids Seminiferous epithelium Sertoli cells Spermatogenesis

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Last Updated: Sep 17, 2025

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Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Published on: September 11, 2014

Area of Science:

Reproductive Biology
Cell Biology
Developmental Biology

Background:

Spermatogenesis, the process of sperm production, is asynchronous, making it difficult to study specific cell types or stages.
Understanding stage-specific functions is crucial for diagnosing male infertility and developing targeted therapies.

Purpose of the Study:

To develop a novel method for analyzing specific stages of spermatogenesis.
To overcome the challenges posed by the asynchronous nature of sperm development.

Main Methods:

Utilized the aldehyde dehydrogenase inhibitor WIN 18,446 to block all-trans retinoic acid (atRA) synthesis.
Prevented the commitment of undifferentiated spermatogonia to meiosis using WIN 18,446.
Administered exogenous atRA after WIN 18,446 treatment to enable stage-specific analysis.

Main Results:

Successfully halted the progression of spermatogenesis at specific points by inhibiting atRA synthesis.
Enabled high-specificity analysis of individual spermatogenesis stages.
Demonstrated a reliable method for dissecting the complexities of sperm development.

Conclusions:

The described method provides a powerful tool for researchers studying spermatogenesis.
This technique allows for detailed investigation of stage-specific and cell-specific functions within the testes.
Offers new avenues for understanding male reproductive health and potential therapeutic targets.