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Updated: Jun 10, 2026
Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants
Published on: August 5, 2021
Enhanced initial proliferation and differentiation of MC3T3-E1 cells on HF/HNO3 solution treated nanostructural
Fuming He1, Feng Zhang, Guoli Yang
1Department of Implantology, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Objective:
The aim of this study was to investigate the effects of nano- or submicro topography and fluoride ion on the biology of osteoblasts.
Study Design:
Pure Ti plates were sandblasted, etched with an HCl/H(2)SO(4) solution (control surface) and then etched in a diluted HF/HNO(3) solution (test surface). MC3T3-E1 cells attached, spread, and proliferated on both surfaces.
Results:
The alkaline phosphatase activity was evidently higher for the test surface than for control surface after 4 and 7 days of cell culture. Real-time PCR showed significant increases in type I collagen and osteocalcin gene expression in osteoblast growth on the test surface after 4 days of culture compared with the control surface.
Conclusion:
With nanotopography and fluoride, hydrogen ions might improve MC3T3-E1 cell proliferation and differentiation during the early stages of cell culture.
