Intracellular tetanization with hyperpolarizing currents potentiates synapses formed by mossy fibers on pyramidal...

Studies on living rat hippocampal slices using point recording in the whole cell configuration addressed the efficiency of the synaptic responses of pyramidal neurons in field CA3 in conditions of minimal stimulation of mossy fibers. Paired-pulse responses were recorded before and after intracellular tetanizing hyperpolarization of pyramidal neurons. In these conditions, potentiation of excitatory synaptic transmission lasting at least 20 min was seen. This phenomenon, termed hyperpolarizing tetanization-induced long-term potentiation, could arise without simultaneous mossy fiber stimulation and showed signs of having a presynaptic origin. Administration of a Ca2+ chelator into pyramidal neurons completely suppressed this potentiation. The results obtained from these experiments suggest that induction of long-term potentiation evoked by hyperpolarizing tetanization was postsynaptic, while its expression appeared to be presynaptic. These results provide evidence of the importance of gamma-rhythm hyperpolarizing oscillations in altering the efficiency of synaptic inputs and the role of its network organization in the mechanisms of cellular plasticity.