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Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Updated: Jun 10, 2026

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

Published on: January 19, 2018

Scanning tunneling spectroscopy.

Harold J W Zandvliet1, Arie van Houselt

  • 1Physical Aspects of Nanoelectronics and the MESA+ Institute for Nanotechnology, University of Twente, 7500 AE Enschede, The Netherlands. h.j.w.zandvliet@utwente.nl

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|July 20, 2010
PubMed
Summary
This summary is machine-generated.

Scanning tunneling microscopy (STM) now offers atomic-scale surface analysis and dynamics. Recent advances like STM-inelastic electron tunneling spectroscopy (STM-IETS) provide molecular vibrational spectra for enhanced chemical specificity.

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Scanning-probe Single-electron Capacitance Spectroscopy
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Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

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Last Updated: Jun 10, 2026

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

Published on: January 19, 2018

Scanning-probe Single-electron Capacitance Spectroscopy
10:53

Scanning-probe Single-electron Capacitance Spectroscopy

Published on: July 30, 2013

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

Area of Science:

  • Surface science
  • Nanotechnology
  • Atomic force microscopy

Background:

  • Scanning tunneling microscopy (STM) enables atomic-scale surface exploration and manipulation.
  • STM can analyze dynamical processes and physical properties at the nanoscale.
  • Traditional STM lacks chemical specificity for detailed surface analysis.

Purpose of the Study:

  • To review recent advancements in scanning tunneling microscopy (STM).
  • To highlight techniques providing chemical specificity in atomic-scale surface analysis.
  • To illustrate these developments with scholarly examples.

Main Methods:

  • Utilizing scanning tunneling microscopy (STM) for high-resolution imaging.
  • Employing scanning tunneling spectroscopy (STS) to map electronic structure.
  • Implementing STM-inelastic electron tunneling spectroscopy (STM-IETS) for molecular vibrational spectra.

Main Results:

  • STM provides unparalleled spatial resolution for surface studies.
  • Combining STM with STS allows detailed electronic structure mapping.
  • STM-IETS successfully measures single-molecule vibrational spectra, adding chemical specificity.

Conclusions:

  • Recent STM developments significantly enhance surface analysis capabilities.
  • STM-IETS offers a powerful tool for probing molecular vibrations and chemical identity.
  • These advancements open new avenues for atomic-scale chemical and physical investigations.