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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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Chromatography in industry.

Peter Schoenmakers¹

¹Polymer Analysis Group, Faculty of Science, University of Amsterdam, 1018 WV Amsterdam, The Netherlands. p.j.schoenmakers@uva.nl

Annual Review of Analytical Chemistry (Palo Alto, Calif.)

|July 20, 2010

Summary

This summary is machine-generated.

Industrial chromatography research, particularly in healthcare, emphasizes robust, automated, and rapid methods. Key trends include hyphenated techniques, multidimensional separations, and parallel processing for high-throughput analysis.

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Area of Science:

Analytical Chemistry
Separation Science

Background:

Industry, especially pharmaceutical and biotech sectors, heavily influences chromatography research.
Industrial research operates collaboratively with academia and other institutions.

Purpose of the Study:

To review industrial chromatography research from 2006 to mid-2008.
To identify key trends and technological adoptions in industrial chromatography.

Main Methods:

Literature review of industry-sponsored or industry-cooperative chromatography research.
Analysis of publications focusing on practical applications and technological advancements.

Main Results:

Widespread adoption of hyphenated techniques (e.g., on-line sample preparation with mass spectrometry).
Increasing use of multidimensional separation methods for complex samples.
Focus on robust, reliable, rapid, and automated chromatography systems.
Exploration of parallel processing and high-throughput analysis strategies.

Conclusions:

Industry drives innovation in chromatography, prioritizing practical, efficient, and automated solutions.
Emerging technologies are readily adopted when they enhance robustness and speed.
The trend is towards integrated and high-throughput analytical workflows.