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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
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Chemical cytometry: fluorescence-based single-cell analysis.

Daniella Cohen1, Jane A Dickerson, Colin D Whitmore

  • 1Department of Chemistry, University of Washington, Seattle, 98195, USA.

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|July 20, 2010
PubMed
Summary
This summary is machine-generated.

Chemical cytometry enables comprehensive single-cell analysis by characterizing numerous components, including nucleic acids, proteins, and metabolites, reaching yoctomole detection levels.

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Sample Preparation for Mass Cytometry Analysis
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Sample Preparation for Mass Cytometry Analysis
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Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Cytometry traditionally analyzes single-cell composition using flow and image techniques with antibody stains.
  • These methods are limited to characterizing only a few components per cell.
  • A need exists for methods analyzing a broader range of cellular components.

Purpose of the Study:

  • To introduce and describe the capabilities of chemical cytometry for comprehensive single-cell analysis.
  • To highlight the advanced analytical tools used in chemical cytometry.
  • To demonstrate the potential for high-sensitivity detection of multiple analytes in single cells.

Main Methods:

  • Chemical cytometry utilizes a suite of powerful analytical tools for multi-component analysis.
  • Nucleic acid analysis employs polymerase chain reaction-based amplification.
  • Protein and metabolite analysis utilizes capillary electrophoresis separation and laser-induced fluorescence detection.

Main Results:

  • Chemical cytometry allows for the characterization of a large number of components within single cells.
  • Advanced techniques enable the detection of nucleic acids, proteins, and metabolites.
  • Yoctomole amounts of numerous analytes can now be detected in single cells.

Conclusions:

  • Chemical cytometry offers a significant advancement over traditional methods for single-cell analysis.
  • The developed tools provide unprecedented depth and breadth in characterizing cellular components.
  • This approach opens new avenues for understanding cellular heterogeneity and function at the molecular level.