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In169, a new class 1 integron that encoded bla(IMP-18) in a multidrug-resistant Pseudomonas aeruginosa isolate from

Guillermina Sánchez-Martinez1, Ulises Jesús Garza-Ramos, Fernando Luis Reyna-Flores

  • 1Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México.

Archives of Medical Research
|July 20, 2010
PubMed
Abstract

Insights

This study identified a new metallo-beta-lactamase (MbetaL) gene array, In169, in carbapenem-resistant Pseudomonas aeruginosa. This finding is significant as it marks the first description of the bla(IMP-18) gene in related isolates from different institutions.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Carbapenem resistance in Pseudomonas aeruginosa is a growing concern.
  • Metallo-beta-lactamases (MbetaL) are a key mechanism of carbapenem resistance.
  • MbetaL genes are often found on class 1 integrons, sometimes alongside other resistance genes.

Purpose of the Study:

  • To describe a novel class 1 integron array, In169, containing the bla(IMP-18) gene.
  • To characterize the genetic environment of bla(IMP-18) in a carbapenem-resistant P. aeruginosa isolate.
  • To compare this finding with other bla(IMP)-type producers.

Main Methods:

  • Screening of 26 multiresistant P. aeruginosa clinical isolates for MbetaL production.
  • Detection of MbetaL genes and integrons using PCR and DNA sequencing.
  • Epidemiological typing by pulsed-field gel electrophoresis (PFGE) and Southern hybridization.

Main Results:

  • One P. aeruginosa isolate (5106) produced MbetaL and harbored the IMP-type gene.
  • The novel class 1 integron array In169 contained bla(IMP-18), aadA2 (two copies), and OXA-2 genes.
  • Isolate 5106 showed genetic relatedness to another bla(IMP-18) positive P. aeruginosa isolate from a different hospital.

Conclusions:

  • This study reports the first characterization of the bla(IMP-18) gene within the In169 integron array.
  • The presence of bla(IMP-18) in genetically related isolates from distinct institutions highlights potential dissemination.
  • Further surveillance is needed to understand the spread of such resistance mechanisms.

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