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Identification of Providencia spp. clinical isolates co-producing carbapenemases IMP-27, OXA-24, and OXA-58 in Mexico

Paola Bocanegra-Ibarias1, Josefina Duran-Bedolla2, Jesús Silva-Sánchez2

  • 1Facultad de Medicina/Hospital Universitario "Dr. José Eleuterio González", Servicio de Infectología, Universidad Autónoma de Nuevo León, Monterrey, México.

Diagnostic Microbiology and Infectious Disease
|March 7, 2024
PubMed

Insights

Carbapenem-resistant bacteria like Providencia rettgeri are a growing threat. This study identifies novel combinations of carbapenemase genes in clinical isolates, highlighting increasing antimicrobial resistance challenges.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Providencia rettgeri is an emerging pathogen causing healthcare-associated infections.
  • Carbapenemase production in P. rettgeri compromises carbapenem efficacy.
  • Co-occurrence of multiple resistance determinants is infrequently reported.

Purpose of the Study:

  • To perform molecular characterization of carbapenemase-producing Providencia spp. clinical isolates.
  • To identify novel carbapenemase gene combinations and their genetic context.

Main Methods:

  • Phenotypic screening for imipenem resistance.
  • Molecular identification of carbapenemase genes (blaNDM-1, blaOXA-58 like, blaIMP-27, blaOXA-24/40 like).
  • Plasmid incompatibility group analysis.

Main Results:

  • Out of 23 imipenem-resistant Providencia spp., 21 harbored blaNDM-1.
  • One isolate showed co-production of blaNDM-1 and blaOXA-58 like.
  • A novel P. rettgeri isolate co-produced blaIMP-27, blaOXA-24 like, and blaOXA-58 like.

Conclusions:

  • The study identified diverse carbapenemase gene profiles in Providencia spp. clinical isolates.
  • A unique P. rettgeri strain with a triple carbapenemase co-production was reported for the first time.
  • These findings underscore the escalating challenge of antimicrobial resistance due to complex resistance mechanisms.
Keywords:
CarbapenemaseIMPIncompatibilityNDMProvidencia

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