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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: Jun 10, 2026

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

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High-throughput in vivo vertebrate screening.

Carlos Pardo-Martin¹, Tsung-Yao Chang, Bryan Kyo Koo

¹Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA.

|July 20, 2010

Summary

This study introduces a high-throughput platform for zebrafish chemical and genetic screening. The system enables rapid, cellular-resolution in vivo imaging and laser manipulation of organs for biological research.

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Area of Science:

Biology
Genetics
Neuroscience

Background:

High-throughput screening is crucial for biological discovery.
Zebrafish larvae are a valuable model organism for in vivo studies.
Current methods for in vivo manipulation and imaging can be time-consuming.

Purpose of the Study:

To develop a high-throughput platform for cellular-resolution in vivo chemical and genetic screens.
To enable rapid imaging and laser manipulation of zebrafish organs.
To facilitate studies on processes like retinal axon guidance and neuronal regeneration.

Main Methods:

Automated loading and precise positioning of zebrafish larvae.
High-speed confocal imaging of superficial and deep organs.
Femtosecond laser microsurgery for targeted manipulation.
Integration of imaging and laser manipulation within a 19-second cycle.

Main Results:

Demonstration of a high-throughput platform for zebrafish screening.
Successful cellular-resolution in vivo imaging and laser manipulation.
Application in small-scale screening of retinal axon guidance mutants.
Utility in neuronal regeneration assays with laser microsurgery.

Conclusions:

The developed platform significantly enhances the efficiency of in vivo zebrafish screening.
It allows for rapid, non-damaging manipulation and imaging of internal organs.
This technology is poised to accelerate research in developmental biology, genetics, and neuroscience.