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Updated: Jun 10, 2026

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
Published on: April 27, 2017
Chemical shift assignment and conformational analysis of monoalkylated acylguanidines
Roland Kleinmaier1, Ruth M Gschwind
1Institut für Organische Chemie, Universität Regensburg, D-93053 Regensburg, Germany.
Abstract:
Monoalkylated acylguanidines are important functional groups in many biologically active compounds and additionally applied in coordination chemistry. Yet a straightforward assignment of the individual NH chemical shifts and the acylguanidine conformations is still missing. Therefore, in this study, NMR spectroscopic approaches for the chemical and especially the conformational assignment of protonated monoalkylated acylguanidines are presented. While NOESY and (3)J(H, H) scalar couplings cannot be applied successfully for the assignment of acylguanidines, (4)J(H, H) scalar couplings in (1)H,(1)H COSY spectra allow for an unambiguous chemical shift and conformational assignment. It is shown that these (4)J(H, H) long-range couplings between individual acylguanidinium NH resonances are observed solely across all-trans (w) pathways. Already one cis orientation in the magnetisation transfer pathway leads to signal intensities below the actual detection limit and significantly lower than cross-peaks from (2)J(NH, NH) couplings or chemical exchange. However, it should be noted that also in the case of conformational exchange being fast on the NMR time scale, averaged cross-peaks from all-trans (4)J(H, H) scalar couplings are detected, which may lead at first glance to an incomplete or even wrong conformational analysis.
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