Sodium salicylate and bile acid-induced colonic secretion in the rat

Previous studies by us suggested that luminally administered sodium salicylate blocks dihydroxy-bile acid-induced colonic secretion in the rat. In the present study, an in vivo rat cecal loop technique is employed to compare the effects of luminally administered and parenterally administered sodium salicylate upon chenodeoxycholic acid-induced colonic secretion. In our experiment, inoculation of four mM chenodeoxycholic acid into the rat cecum produced net secretion of water and sodium which was not reversed by preincubation of this bile acid with eight mM of sodium salicylate. Similarly, an intravenous bolus of either five mg of sodium salicylate per kg of body weight or 50 mg of sodium salicylate per kg of body weight failed to block salt or water secretion. Furthermore, 30 minute incubation of chenodeoxycholic acid with sodium salicylate produced neither reduction of in vitro aqueous bile acid concentration nor inhibition of ex vivo bile acid-facilitated hypotonic red cell hemolysis. These data suggest that sodium salicylate fails to sequester bile acids from aqueous solution and fails to block bile acid-mediated colonic secretion in the rat.