Related Experiment Video
Updated: Jun 10, 2026
Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics
Published on: May 17, 2019
Selective culture of different types of human parotid gland cells
Yen-Hui Chan1, Tsung-Wei Huang, Tai-Horng Young
1Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
Background:
Advances in salivary gland tissue engineering can benefit patients diagnosed with xerostomia. Complexity of the gland explains the urgent demand for a reliable protocol to isolate and expand various gland cells that can be used for further study.
Methods:
Three cells with different morphologies were isolated from the same human parotid glands using different culture medium systems and then were identified by the expressions from mRNA to the protein level.
Results:
Among the 34 specimens, parotid gland acinar cells, myoepithelial cells, and fibroblasts expressing specific markers that belonged to individual cell types, were successfully isolated and expanded from 30 specimens without a complex mechanical process and expensive flow technique.
Conclusion:
The proposed protocol is simple with a high success rate to culture various gland cells, making it highly promising for use in future tissue engineering studies.
Related Concept Videos
01:13Exocrine Glands: Types of Secretions
Serous glands produce watery secretions rich in digestive enzymes and proteins. The constituent cells of the serous gland have centrally located nuclei and eosinophilic secretory granules in the cytoplasm. The parotid gland is an example of a serous gland. It secretes saliva, which contains enzymes, such as lipases and...
00:59The Parathyroid Glands
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by producing...