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Biologic markers for odontogenic periradicular periodontitis
Bruna Burgener1, Angelique R Ford, Hongsa Situ
1Department of Endodontics, University of Illinois at Chicago College of Dentistry, Chicago, Illinois 60612, USA.
Journal of Endodontics
|July 22, 2010
Summary
Interleukin-1beta (IL-1beta) and dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) are not reliable markers for apical periodontitis. However, elevated total protein in GCF may indicate periapical disease.
Area of Science:
- Biomarkers
- Dental Diagnostics
- Periodontitis Research
Background:
- Traditional periapical radiographs for diagnosing apical periodontitis can be challenging and may produce false-negative results.
- Investigating novel biomarkers in gingival crevicular fluid (GCF) is crucial for accurate diagnosis.
Purpose of the Study:
- To evaluate interleukin-1beta (IL-1beta) and dentin sialoprotein (DSP) in GCF as potential biomarkers for apical periodontitis.
- To assess the diagnostic utility of these proteins in distinguishing diseased from healthy teeth.
Main Methods:
- GCF samples collected from 40 patients with apical periodontitis and healthy controls.
- Total protein concentration measured using the Bio-Rad protein assay.
- IL-1beta and DSP levels analyzed via enzyme-linked immunosorbent assay (ELISA).
Main Results:
- Significantly higher total protein concentration was observed in the GCF of teeth with apical periodontitis compared to controls.
- No statistically significant difference in IL-1beta or DSP levels was found between the disease and control groups.
Conclusions:
- IL-1beta and DSP in GCF did not prove to be significant biomarkers for apical periodontitis in this study.
- Elevated total protein levels in GCF show potential as a diagnostic marker for periapical disease.