Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 10, 2026

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
08:44

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Published on: July 30, 2020

Labeling lipids for imaging in fixed cells.

Carsten Schultz, Anne B Neef, Theodorus W Gadella

    Cold Spring Harbor Protocols
    |July 22, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Decoding protein-phospholipid interaction networks in cancer: the role of acyl-chain remodeling.

    RSC chemical biology·2026
    Same author

    Side-by-Side Systematic Characterization of Novel FPs in Budding Yeast.

    ACS synthetic biology·2026
    Same author

    High-resolution phosphoinositide analysis.

    Methods in enzymology·2026
    Same author

    An mTurquoise2-Based Glucose Biosensor.

    ACS sensors·2026
    Same author

    An Integrated Method for Profiling Lipid-Protein Interactions Using Multifunctional Lipid Probes.

    bioRxiv : the preprint server for biology·2026
    Same author

    Emerging roles of lipids in the flavivirus life cycle.

    Trends in microbiology·2026
    Same journal

    High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

    Cold Spring Harbor protocols·2025
    Same journal

    Grain Quality in Maize.

    Cold Spring Harbor protocols·2025
    Same journal

    High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

    Cold Spring Harbor protocols·2025
    Same journal

    Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

    Cold Spring Harbor protocols·2025
    Same journal

    The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

    Cold Spring Harbor protocols·2025
    Same journal

    Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

    Cold Spring Harbor protocols·2025
    See all related articles

    Researchers developed a new method to directly label lipids in fixed cells. This technique allows for accurate lipid localization analysis, overcoming limitations of previous fluorescent tagging methods for intracellular signaling studies.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Molecular Imaging

    Background:

    • Fluorescently tagged lipid-binding domains are used to image lipids in intracellular signaling.
    • Current methods often fail to detect lipids like phosphatidylinositol 4,5-bisphosphate (PIP(2)) in the endoplasmic reticulum or Golgi.
    • Existing techniques are primarily effective for lipids in the plasma membrane.

    Purpose of the Study:

    • To develop an alternative method for localizing specific lipids within cells.
    • To enable direct fluorescent labeling of lipids in fixed cells for location analysis.
    • To overcome the limitations of current lipid-imaging techniques.

    Main Methods:

    • Development of a novel protocol for direct lipid labeling.
    • Utilizing minimally modified lipid derivatives for fluorescent tagging.

    More Related Videos

    LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium
    06:16

    LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium

    Published on: July 28, 2023

    Related Experiment Videos

    Last Updated: Jun 10, 2026

    Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
    08:44

    Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

    Published on: July 30, 2020

    LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium
    06:16

    LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium

    Published on: July 28, 2023

  • Employing a single, specific chemical reaction for labeling.

    • Main Results:

    • Successful direct fluorescent labeling of lipids in fixed cells.
    • Demonstrated accurate lipid localization analysis using the new method.
    • Overcame limitations of previous methods for detecting specific lipids in organelle membranes.

    Conclusions:

    • The developed protocol offers a new approach for studying lipid localization.
    • This method enhances the ability to image and analyze lipids involved in intracellular signaling.
    • Provides a valuable tool for cell biology research, particularly for organelle lipid analysis.