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Updated: Jun 10, 2026

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
Published on: September 1, 2009
Aneuploidies in embryos and spermatozoa from patients with Y chromosome microdeletions
Emilia Mateu1, Lorena Rodrigo, M Carmen Martínez
1Instituto Valenciano de Infertilidad, Valencia, Spain. emateu@ivi.es
Abstract:
In patients with Y chromosome microdeletions and high percentage of numeric chromosome abnormalities detected by fluorescence in situ hybridization on sperm, a high percentage of abnormal embryos was observed compared with oligozoospermic patients without Y chromosome microdeletions, with a significant increase in the percentage of embryos with monosomy X. Differences in fertilization rates between the different patient groups were not observed; however, blastocyst rates were significantly impaired in patients with Y chromosome microdeletions.
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