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Related Experiment Video

Updated: Jun 10, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

Efficient industrial dye decolorization by Bacillus sp. VUS with its enzyme system.

Vishal V Dawkar¹, Umesh U Jadhav, Dhawal P Tamboli

¹Department of Biochemistry, Shivaji University, Kolhapur 416004, India. vvdawkar@gmail.com

Ecotoxicology and Environmental Safety

|July 27, 2010

Summary

Bacillus sp. VUS effectively decolorized Orange T4LL dye in 24 hours using enzymes like lignin peroxidase and reductases. The non-toxic degradation products are suitable for textile effluent treatment.

Related Experiment Videos

Last Updated: Jun 10, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

Area of Science:

Environmental Microbiology
Biotechnology
Industrial Chemistry

Background:

Industrial dyes pose environmental challenges.
Textile effluents require effective treatment methods.
Microbial decolorization offers a sustainable solution.

Purpose of the Study:

To investigate the role of enzymes in Orange T4LL dye decolorization by Bacillus sp. VUS.
To characterize the degradation products and assess their environmental impact.
To evaluate the potential of Bacillus sp. VUS for textile wastewater treatment.

Main Methods:

Static anoxic decolorization assay.
Enzyme activity assays (lignin peroxidase, tyrosinase, reductases).
Spectroscopic and chromatographic monitoring (UV-Vis, FTIR, HPLC, GC-MS).
Phytotoxicity, Chemical Oxygen Demand (COD), and Biological Oxygen Demand (BOD) tests.

Main Results:

Bacillus sp. VUS decolorized Orange T4LL within 24 hours.
Significant induction of lignin peroxidase, tyrosinase, and reductase activities observed.
Biodegradation products identified as 4-methyl-2-o-tolylazo-benzene-1,3-diamine and [3-(phenyl-hydrazono)-cyclohexa-1,4-dienyl]-methanol.
Degradation products showed no toxicity to Triticum aestivum and Sorghum bicolor, with favorable COD and BOD levels.

Conclusions:

Bacillus sp. VUS possesses an effective enzyme system for Orange T4LL decolorization.
The microbial degradation process yields non-toxic byproducts.
This bacterium shows promise as a biological tool for treating textile industry effluents.