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Updated: Jun 10, 2026
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Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
Published on: September 21, 2019
Filtration assay for arachidonic acid release.
Pauli M Turunen1, Jaana Putula, Jyrki P Kukkonen
1Biochemistry and Cell Biology, Department of Veterinary Biosciences, University of Helsinki, FIN-00014 Helsinki, Finland.
Analytical Biochemistry
|July 27, 2010
Summary
We developed a fast, reproducible method to measure arachidonic acid release in cell signaling studies. This new assay saves time and effort, enabling larger experimental series for ligand screening.
Area of Science:
- Cellular Biology
- Biochemistry
- Pharmacology
Background:
- Arachidonic acid (AA) release is a critical cellular signaling event.
- Current methods for measuring fatty acid release are manual, laborious, and time-consuming.
Purpose of the Study:
- To develop a fast, reproducible, and high-throughput method for assessing arachidonic acid release.
- To facilitate agonist concentration-response studies and ligand screening.
Main Methods:
- A novel assay utilizing 96-well filter plates and cells in suspension.
- Measurement of radioactivity release from prelabeled cells.
Main Results:
- The new method significantly reduces time and effort compared to traditional assays.
- The assay is suitable for large experimental series, previously unattainable for AA release studies.
- Demonstrates high reproducibility and suitability for agonist concentration-response studies.
Conclusions:
- This method provides a substantial advancement for studying arachidonic acid release in cell signaling.
- Enables efficient and scalable ligand screening and pharmacological studies.
