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Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study

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Triterpenoid alkaloids from Buxus microphylla.

Yu-Xin Yan¹, Jian-Chao Chen, Yun Sun

¹State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, P. R. China.

Chemistry & Biodiversity

|July 27, 2010

Summary

This summary is machine-generated.

Two new triterpenoid alkaloids, buxmicrophyllines J and K, were isolated from Buxus microphylla. Compound 6 demonstrated significant cytotoxicity against three human cancer cell lines.

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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

Natural Product Chemistry
Medicinal Chemistry

Background:

Buxus microphylla is a plant source of bioactive compounds.
Triterpenoid alkaloids represent a class of natural products with diverse biological activities.

Purpose of the Study:

To isolate and characterize new triterpenoid alkaloids from Buxus microphylla.
To evaluate the cytotoxic activity of isolated compounds against human cancer cell lines.

Main Methods:

Isolation of compounds using chromatographic techniques.
Structure elucidation utilizing Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS).
Cytotoxicity assays against HL-60, SMMC-7721, A-549, SK-BR-3, and PANC-1 cell lines.

Main Results:

Two new triterpenoid alkaloids, buxmicrophyllines J and K, were identified.
Four known analogues were also isolated and characterized.
Compound 6 exhibited significant cytotoxicity against HL-60, SK-BR-3, and PANC-1 cell lines, with IC50 values ranging from 6.46 to 28.57 µM.

Conclusions:

Buxus microphylla is a valuable source of novel bioactive triterpenoid alkaloids.
Compound 6 shows promising cytotoxic potential for further investigation in cancer therapy.