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Genetic and functional interactions between Mus81-Mms4 and Rad27

Min-Jung Kang1, Chul-Hwan Lee, Young-Hoon Kang

  • 1Center for DNA Replication and Genome Instability, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.

Nucleic Acids Research
|July 28, 2010
PubMed

Insights

The study reveals that Mus81-Mms4 endonuclease suppresses dna2 mutations by interacting with Rad27 (yeast Fen1). This interaction stimulates Rad27 activity, aiding DNA replication fork processing and resolving replication impediments.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Rad27 (yeast Fen1) and Dna2 are key endonucleases for Okazaki fragment processing in yeast.
  • Mus81-Mms4 is a structure-specific endonuclease involved in resolving stalled replication forks and recombination intermediates.

Purpose of the Study:

  • To investigate the functional and physical interaction between Mus81-Mms4 and Rad27.
  • To elucidate the role of this interaction in suppressing dna2 mutational defects and its impact on DNA replication.

Main Methods:

  • In vitro assays to assess the stimulation of Rad27 and Mus81-Mms4 activities.
  • Analysis of protein-protein interactions, specifically involving the C-terminal fragment of Rad27.
  • Genetic analysis of double mutants (rad27 mus81, rad27 mms4) to evaluate synergistic lethality.

Main Results:

  • Mus81-Mms4 significantly stimulates Rad27 activity, restoring growth in dna2 mutants.
  • Rad27 stimulates Mus81-Mms4 cleavage of various substrates, including regressed replication forks.
  • Rad27's stimulation of Mus81-Mms4 is mediated by a protein-protein interaction, independent of Rad27's catalytic activity, requiring its C-terminal 64 amino acids.

Conclusions:

  • Mus81-Mms4 and Rad27 collaborate in DNA replication to resolve structures that impede replication progression.
  • The synergistic lethality of rad27 mus81 and rad27 mms4 double mutants underscores the critical importance of their combined function.
  • The interaction network involving Rad27, Dna2, and Mus81-Mms4 is crucial for maintaining genomic stability during DNA replication.

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