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Perfluorochemical emulsions decrease Kupffer cell phagocytosis.

L A Bottalico1, H T Betensky, Y B Min

  • 1Department of Chemistry, Barnard College, Columbia University, New York, New York 10027.

Hepatology (Baltimore, Md.)
|July 1, 1991
PubMed
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Perfluorochemical emulsions can affect the reticuloendothelial system. While Fluosol-DA showed minimal impact on clearance, Oxypherol-ET temporarily reduced liver and spleen function in rats.

Area of Science:

  • Biomedical Engineering
  • Pharmacology
  • Toxicology

Background:

  • Perfluorochemical (PFC) emulsions are investigated as blood substitutes.
  • A key concern is their clearance by the reticuloendothelial system (RES), mainly the liver and spleen.
  • Understanding RES function post-PFC administration is crucial for safety assessment.

Purpose of the Study:

  • To evaluate the impact of two PFC emulsions, Fluosol-DA and Oxypherol-ET, on RES clearance functions.
  • To compare the efficacy of an isolated perfused liver model versus in vivo studies for assessing RES function.
  • To determine the effect of PFCs on the phagocytosis of colloidal carbon and sheep red blood cells.

Main Methods:

  • Male rats were administered Fluosol-DA or Oxypherol-ET for four consecutive days.

Related Experiment Videos

  • RES clearance was assessed using colloidal carbon (<1 micron) and 51Cr-sheep red blood cells (~8 microns).
  • Experiments were conducted both in vivo and using an isolated perfused liver model, with sample collection over 1 hour.

    • Main Results:

    • The isolated perfused liver model accurately reflected in vivo RES clearance rates.
    • Oxypherol-ET significantly depressed carbon clearance (up to 67%) in the perfused liver and in vivo (74%) one day post-treatment.
    • Fluosol-DA showed minimal impact on carbon clearance, while both PFCs temporarily reduced sheep red blood cell clearance.
    • Kupffer cell phagocytosis was affected, but overall RES function remained largely intact.

    Conclusions:

    • The isolated perfused liver serves as a reliable model for evaluating liver clearance function.
    • While certain perfluorochemical emulsions can transiently impact RES phagocytosis, they do not significantly compromise overall RES function at the tested doses.
    • Fluosol-DA appears to have a more favorable RES interaction profile compared to Oxypherol-ET.