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Crystalline inclusion bodies in rabbit embryos
Journal of Embryology and Experimental Morphology
|April 1, 1978
Summary
Crystalline inclusion bodies (CIB) in rabbit embryos form from granular vesicles. The uterus is essential for CIB development, as they do not form when embryos are prevented from reaching it.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Cell Biology
Background:
- Crystalline inclusion bodies (CIB) are ultrastructural components observed in rabbit trophoblast cells and uterine endometrium.
- The origin and developmental timing of CIB in early rabbit embryos are not fully understood.
Purpose of the Study:
- To describe the developmental stages of CIB formation in rabbit embryos.
- To correlate CIB development with embryonic age.
- To determine the necessity of the uterus for CIB formation in embryos.
Main Methods:
- Observation of rabbit embryos at various developmental stages using electron microscopy.
- Surgical manipulation to prevent embryos from reaching the uterus (tube-locking).
- Transplantation of tube-locked embryos into the uterus to assess CIB development.
Main Results:
- CIB are first detected in embryos at 4 days 6 hours post coitum, becoming numerous by 4.5 days.
- Granular vesicles, precursors to CIB, are present from 2.5 days and decrease as CIB increase.
- CIB formation is inhibited in embryos prevented from entering the uterus but occurs within 36 hours after uterine transplantation.
Conclusions:
- Crystalline inclusion bodies in rabbit embryos originate from granular vesicles.
- The uterine environment is essential for the formation of crystalline inclusion bodies in rabbit embryos.