Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microfluidic patch integrated with cobalt oxide/cobalt phosphate nanozyme for electrochemical lactate sensing at neutral pH.

Talanta·2026
Same author

A Polydopamine-Based Molecularly Imprinted Electrochemical Sensor for Fentanyl Determination.

ACS omega·2025
Same author

Rapid assembly of mixed thiols for toll-like receptor-based electrochemical pathogen sensing.

Analytical methods : advancing methods and applications·2024
Same author

Integration of complementary split-ring resonators into digital microfluidics for manipulation and direct sensing of droplet composition.

Lab on a chip·2024
Same author

Electrochemical Determination of Fentanyl Using Carbon Nanofiber-Modified Electrodes.

ACS omega·2024
Same author

Celebrating the 30th anniversary of a pioneering microfluidics paper.

Lab on a chip·2023
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Related Experiment Video

Updated: Jun 10, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Microfluidic devices for electrokinetic sample fractionation.

Zhen Wang1, Justine Taylor, Abebaw B Jemere

  • 1Department of Chemistry, University of Alberta, Edmonton, AB, Canada.

Electrophoresis
|July 29, 2010
PubMed
Summary
This summary is machine-generated.

We developed microchip-based devices for in-space sample fractionation in proteomics. These systems efficiently separate and collect samples without contamination, proving useful for future proteomic platforms.

More Related Videos

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Related Experiment Videos

Last Updated: Jun 10, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Space Science

Background:

  • Proteomics research requires efficient sample handling and separation.
  • In-space applications demand robust and miniaturized analytical tools.
  • Previous microfluidic devices faced challenges in high-throughput fractionation and contamination control.

Purpose of the Study:

  • To develop and demonstrate microchip-based sample fractionators and collectors for in-space proteomics.
  • To evaluate the performance of three generations of microchip designs.
  • To ensure high-throughput, contamination-free sample processing.

Main Methods:

  • Design and fabrication of microfluidic chips with integrated capillary electrophoresis (CE) and fractionation channels.
  • Utilizing sheath streams for contamination control during sample transfer.
  • Sequential electrokinetic driving of CE-separated analytes to multiple collection channels.
  • Testing with protein mixtures (IgG and BSA) and subsequent on-chip CE analysis for purity verification.

Main Results:

  • Demonstrated sequential collection of CE-separated samples in multiple channels without cross-contamination.
  • A 36-channel fractionator showed high-throughput capability with no observed cross-contamination.
  • Achieved efficient and reproducible non-contaminated sample collection over 15 injections.
  • Experimental results closely matched PSpice simulations for flow behavior and contamination control.

Conclusions:

  • Microchip-based fractionators and collectors are effective for in-space proteomics sample preparation.
  • The developed technology offers efficient, reproducible, and contamination-free sample handling.
  • This technology holds significant potential for integration into future space-based proteomic platforms.