[Soluble proteins of the plerocercoid of Spirometra mansoni]

To analyze soluble proteins of the plerocercoid of Spirometra mansoni.

The total protein of the plerocercoid of S. mansoni was separated by SDS-PAGE and two-dimensional electrophoresis (2-DE). Western blotting was performed to find out distinct antigens by sera of plerocercoid-infected mice.

A total of 33 protein bands were separated by SDS-PAGE (Mr 13,800-145,400). Four of them were high-abundance proteins with Mr 26,500, Mr 37,600, Mr 88,200, and Mr 130,200, respectively. At least two protein bands of Mr 31,600 and 37,600 reacted with the infected mice sera. 367 protein spots were detected on 2-DE gel, among which. about 67% proteins were found within Mr 18,840-46,800 and isoelectric point (pI) 4-7. Western blotting showed 30 antigen spots specifically reacting with sera from mice infected by S. mansoni.

Two protein bands and thirty protein spots are specific acidic proteins of S. mansoni plerocercoid.