Synthesis and properties of triplex-forming oligonucleotides containing
Chenguang Lou1, Qiang Xiao, Lavinia Brennan
1School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
Bioorganic & Medicinal Chemistry
|August 3, 2010
Abstract:
2'-O-(2-Methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine phosphoramidite (MEPU) has been synthesized from d-ribose and 5-iodouracil and incorporated into triplex-forming oligonucleotides (TFOs) by automated solid-phase oligonucleotide synthesis. The TFOs gave very high triplex stability with their target duplexes as measured by ultraviolet/fluorescence melting and DNase I footprinting. The incorporation of MEPU into TFOs renders them resistant to degradation by serum nucleases.
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