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Updated: Jun 10, 2026
Tissue Engineering of the Intestine in a Murine Model
Published on: December 1, 2012
Porcine peritoneum as source of biocompatible collagen in mice
Cristina Jardelino1, Esther Rieko Takamori, Luiz Felipe Hermida
1Program in Dentistry, Dental School, UFF, Rio de Janeiro, Brazil.
Purpose:
To investigate the biocompatibility and biodegradability of a membrane made from porcine peritoneum.
Methods:
The membrane (5x5 mm) was inserted in the subcutaneous tissue on the back of 15 mice, which were killed after 1, 3 and 9 weeks (ISO 10993-6). The cellular components of the inflammatory response and degradation of the membrane were analyzed in hematoxylin-eosin-stained histological sections.
Results:
After one week, mononuclear cells were observed inside the membrane. After three weeks, the material was almost completely absorbed. After nine weeks, there was no presence of material and there were signs of tissue remodeling. There was neither a foreign body reaction nor signs of tissue necrosis.
Conclusion:
The collagen membrane derived from porcine peritoneum is biocompatible and bioabsorbable when implanted in the subcutaneous tissue of mice.
Insights
This study shows that a collagen membrane from porcine peritoneum is safe and breaks down naturally in mice. The material was well-tolerated, indicating its potential for tissue regeneration applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Veterinary Medicine
Background:
- Biocompatible and biodegradable materials are crucial for medical implants.
- Porcine peritoneum is a potential source for collagen-based biomaterials.
Purpose of the Study:
- To evaluate the biocompatibility and biodegradability of a porcine peritoneum-derived membrane.
- To assess the host tissue response to the implanted membrane in a preclinical model.
Main Methods:
- A porcine peritoneum membrane (5x5 mm) was implanted subcutaneously in 15 mice.
- Histological analysis using hematoxylin-eosin staining was performed at 1, 3, and 9 weeks post-implantation.
- Evaluation followed ISO 10993-6 guidelines for biological evaluation of medical devices.
Main Results:
- Mononuclear cell infiltration observed within the membrane at 1 week.
- Near-complete absorption of the membrane by 3 weeks.
- Absence of the material and evidence of tissue remodeling by 9 weeks.
- No significant foreign body reaction or tissue necrosis detected.
Conclusions:
- The collagen membrane derived from porcine peritoneum demonstrates excellent biocompatibility.
- The material is effectively bioabsorbable when implanted in subcutaneous tissue.
- These findings support its potential use in regenerative medicine and tissue repair.
