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Related Concept Videos

Spontaneous and Induced Mutations01:30

Spontaneous and Induced Mutations

Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).
Mutations in Microorganisms01:18

Mutations in Microorganisms

Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair01:36

Mismatch Repair

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Updated: Jun 10, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

Random mutagenesis using a mutator strain.

Ghazala Muteeb1, Ranjan Sen

  • 1Laboratory of Transcription Biology, Center for DNA Fingerprinting and Diagnostics, Hyderabad, India.

Methods in Molecular Biology (Clifton, N.J.)
|August 3, 2010
PubMed
Summary
This summary is machine-generated.

Random mutagenesis using Escherichia coli XL1-red generates valuable plasmid libraries for gene function studies. This cost-effective method introduces random point mutations, aiding in the discovery of mutant phenotypes.

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Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
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Last Updated: Jun 10, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
04:51

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Published on: November 14, 2016

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Random mutagenesis is crucial for elucidating gene product functions.
  • Mutator strains facilitate the generation of diverse genetic variations.

Purpose of the Study:

  • To describe a method for creating randomly mutagenized plasmid libraries.
  • To enable the study of gene functions through mutant screening.

Main Methods:

  • Utilizing the mutator strain Escherichia coli XL1-red for plasmid propagation.
  • Introducing random point mutations into cloned genes via this strain.
  • Generating and screening mutagenized plasmid libraries for desired phenotypes.

Main Results:

  • A simple and economical approach for generating libraries with a high mutation rate.
  • Successful production of randomly mutagenized plasmid libraries.
  • Facilitation of mutant phenotype screening.

Conclusions:

  • The XL1-red strain provides an efficient tool for random mutagenesis.
  • This technique is valuable for functional genomics and genetic research.
  • The method supports the economic generation of diverse mutant libraries.