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Published on: April 5, 2019
Polymeric ionic liquid as a dynamic coating additive for separation of basic proteins by capillary electrophoresis
Jing Li1, Haifeng Han, Qing Wang
1Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
Analytica Chimica Acta
|August 4, 2010
Summary
A new capillary electrophoresis method uses a polymeric ionic liquid (PIL) to coat capillaries, improving the separation of basic proteins. This technique offers stable flow and prevents protein adsorption for efficient analysis.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- Analysis of basic proteins can be challenging due to wall adsorption.
- Polymeric ionic liquids (PILs) offer unique properties for surface modification.
Purpose of the Study:
- To develop a simple, economical, and effective CE method for analyzing basic proteins.
- To utilize a polymeric ionic liquid as a dynamic coating additive in CE.
- To improve separation efficiency and reduce protein adsorption in CE.
Main Methods:
- Developed a CE method using poly(1-vinyl-3-butylimidazolium) bromide as a dynamic coating.
- Established a cationic coating on fused-silica capillaries using PIL.
- Optimized PIL concentration, background electrolyte pH, and concentration.
Main Results:
- Achieved stable reversed electroosmotic flow.
- Effectively eliminated wall adsorption of basic proteins.
- Obtained high peak efficiencies (247,000–540,000 plates/m) within 11 minutes.
- Demonstrated excellent run-to-run reproducibility (RSDs < 0.37%).
Conclusions:
- The PIL-modified capillary electrophoresis method provides efficient and reproducible separation of basic proteins.
- This method is a simple and economical approach for protein analysis.
- The developed technique overcomes common challenges in basic protein CE analysis.
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