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Published on: December 30, 2025
Electrogenerated chemiluminescence biosensor for glucose based on poly(luminol-aniline) nanowires composite modified
Guixin Li1, Jinlan Lian, Xingwang Zheng
1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Engineering Research Center of Historical and Cultural Heritage Protection, Ministry of Education, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, PR China.
Abstract:
Poly(luminol-aniline) nanowires composite (PLANC) was synthesized on the surface of graphite electrode by electro-oxidizing the mixture of luminol with aniline in the H2SO4 acidic medium. The properties of the nanowires composite were characterized by transmission electron microscopy, electrogenerated chemiluminescence and electrochemical impedance spectroscopy. The investigating results showed that: firstly, while the luminol alone was electro-oxidized on the surface of the graphite electrode with the proposed synthetic method, only the polyluminol film was formed on electrode surface. However, while a suitable amount of the aniline was present in the luminol solution, the PLANC could be formed on the electrode surface and this PLANC presented better ECL properties for H2O2 compared with pure polyluminol film; secondly, since the PLANC modified electrode not only provided a larger surface area for higher glucose enzyme loading but also formed the nano-structured interface on the electrode surface to improve the analytical performances of the electrogenerated chemiluminescence (ECL) biosensor for glucose, the resulting ECL biosensor presented good response to glucose and a novel ECL biosensor for glucose was proposed.
