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Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Related Experiment Video

Updated: Jun 10, 2026

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells
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Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Published on: May 23, 2025

Cellular imaging using matrix-enhanced and metal-assisted SIMS.

A F Maarten Altelaar1, Sander R Piersma

  • 1Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Utrecht, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|August 4, 2010
PubMed
Summary
This summary is machine-generated.

Imaging mass spectrometry (IMS) reveals molecular details in cells. Techniques like matrix-enhanced (ME) and metal-assisted (MetA) SIMS enable high-resolution imaging of lipids and sterols in biological samples.

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Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
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Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells
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Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
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Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix

Published on: December 22, 2011

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology

Background:

  • Imaging mass spectrometry (IMS) provides direct molecular and spatial information from biological surfaces.
  • Secondary Ion Mass Spectrometry (SIMS) is a powerful technique for molecular analysis.
  • Understanding cellular and tissue molecular composition is crucial for biological research.

Purpose of the Study:

  • To detail methods for retrieving molecular information from tissue sections using ME-SIMS and MetA-SIMS.
  • To demonstrate the capability of these techniques for high-resolution cellular imaging.
  • To investigate the spatial distribution of membrane components in neuroblastoma cells.

Main Methods:

  • Surface metallization via plasma coating for enhanced desorption/ionization in imaging time-of-flight (ToF) SIMS.
  • Application of matrix-enhanced (ME) SIMS using 2,5-dihydroxybenzoic acid.
  • Metal-assisted (MetA) SIMS for analyzing membrane components like lipids and sterols.

Main Results:

  • High-resolution images of cholesterol and other membrane components were obtained from single neuroblastoma cells.
  • Subcellular details of membrane composition were visualized.
  • Intact molecular ion imaging of phosphatidylcholine (PC) and sphingomyelin (SM) was achieved at the cellular level using ME-SIMS.

Conclusions:

  • ME-SIMS and MetA-SIMS are effective for direct molecular imaging of biological surfaces.
  • These techniques provide high-resolution spatial information on cellular and subcellular molecular distributions.
  • The methods enhance the understanding of lipid and sterol localization within cells.