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[Study on sequence characterized amplified region (SCAR) markers in Dendrobium candidum]
Bo Jin1, Fu-Sheng Jiang, Jing Yu
1Zhejiang Chinese Medical University, Hangzhou 310053, China. jinbont@sina.com
Objective:
To establish an effective way for rapid identification of Dendrobium candidum based on DNA molecular marker.
Methods:
The genetic diversity among 11 wild species of Dendrobium was studied by using the random amplification polymorphism DNA. The special segment with random primer in Dendrobium candidum was recovered, cloned and sequenced. According to its sequence, a pair of specific primers was synthesized and tested for the specific detection of Dendrobium candidum.
Results:
The results of PCR showed that only a 302 bp electrophoresis band of Dendrobium candidum named DS-302 was found. According to the result of sequence analysis in the Genbank databases, no distinct comparability was found. And a specific primer was designed and used to identify Dendrobium candidum from other Dendrobium species effectively. There was a same band like DS-302 in Dendrobium candidum, but not be discovered in other species of Dendrobium.
Conclusions:
The RAPD marker DS-302 was successfully converted into SCAR marker. It was an effective way to identify Dendrobium candidum more rapidly.
