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[Fingerprint analysis of Dipsacus asperoides by HPLC]
Yong Liu1, Ying-Fang Wei, Jun-Ping Zhu
1Chengdu University of TCM, Chengdu 611137, China. lys372@163.com
Objective:
To establish the HPLC fingerprints of Dipsacus asperoides for reflecting the internal information, evaluating its internal quality.
Methods:
20 batches of D. asperoides were collected from different place with the HPLC fingerprints method. Chromatographic column: Welchrom-C18 (250 mm x 4. 6 mm, 5 microm), mobile phase: acetonitrile and water (gradient elution), flow rate: 1.0 mL/min, detection wavelength: 212 nm, column temperature: 35 degrees C.
Results:
The common mode of HPLC fingerprint was established and similar degrees to D. asperoides from different areas were compared.
Conclusion:
The method is stable, reliable, and with full information which can be used for quality evaluation, quality control item and crude drug identification of D. asperoides.
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