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Related Concept Videos

Bone Remodeling and Repair01:31

Bone Remodeling and Repair

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
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Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
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Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...

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Bone morphogenetic protein-7 enhances cementoblast function in vitro.

Sema S Hakki1, Brian L Foster, Kanako J Nagatomo

  • 1Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya, Turkey. sshakki@yahoo.com

Journal of Periodontology
|August 5, 2010
PubMed
Summary
This summary is machine-generated.

Bone morphogenetic protein-7 (BMP-7) significantly enhances cementoblast activity, promoting biomineralization and regulating key genes for periodontal regeneration. This suggests BMP-7

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Area of Science:

  • Cell Biology
  • Regenerative Medicine
  • Biochemistry

Background:

  • Bone morphogenetic protein (BMP)-7 is a known bone-inducing factor.
  • Its specific effects on cementoblasts, crucial for tooth root formation, were previously undefined.
  • This study investigates BMP-7's impact on cementoblasts and their associated gene expression.

Purpose of the Study:

  • To determine the effect of BMP-7 on cementoblast gene expression, focusing on mineralized tissue markers, extracellular matrix (ECM), and cell adhesion molecules (CAMs).
  • To assess BMP-7's influence on cementoblast-mediated biomineralization in vitro.

Main Methods:

  • Culturing a murine immortalized cementoblast cell line (OCCM.30) with and without BMP-7.
  • Analyzing mRNA levels of bone/cementum markers (BSP, OCN, OPN, Runx2) using real-time quantitative reverse transcription-polymerase chain reaction (Q-PCR).
  • Assessing in vitro mineral nodule formation and using gene-expression arrays for ECM and CAM genes.

Main Results:

  • BMP-7 significantly increased BSP, OCN, OPN, and Runx2 mRNA expression in cementoblasts.
  • BMP-7 markedly stimulated cementoblast-mediated biomineralization in vitro.
  • BMP-7 altered the expression profile of ECM and CAM genes, including increased MMPs and decreased TIMPs.

Conclusions:

  • BMP-7 effectively regulates mineralized tissue-associated genes and enhances biomineralization in cementoblasts.
  • These findings highlight BMP-7's potential for periodontal tissue regeneration therapies.
  • Further research into BMP-7's role in periodontal regeneration is warranted.