Related Experiment Video
Updated: Jun 10, 2026
Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research
Published on: September 15, 2023
Automated assessment of tumour microvascular architecture in vivo
Sven Becker1, Sebastian Strieth, Martin Canis
1Department of Otorhinolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Marchioninistrasse 15, D-81377 Munich, Germany. sven.becker@med.uni-muenchen.de.
Background:
Quantitative analysis of tumour angiogenesis is an indispensable prerequisite for the development of novel antivascular treatment strategies. The aim of this study was to develop an automated analysis technique enabling quantitative assessment of complete tumour vascular networks non-invasively in vivo.
Materials And Methods:
Experiments were performed in A-Mel-3 implanted in transparent dorsal skinfold chambers of the Syrian Golden hamster. Tumours were imaged in two dimensions by means of a computer controlled scanning-table combined with intravital microscopy. Functional vessel density was automatically quantified online with a digital imaging application package.
Results:
Data for functional vessel density correlated excellently with data obtained by off-line video-based frame-by-frame analysis (R((S))=0.947, r(2)=0.959, n=48).
Conclusion:
This new technique enables automated, quantitative assessment of complete tumour vascular networks and therefore provides a promising tool to characterise changes of the entire tumour vascularity in response to antivascular treatment regimes.
