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Flow Cytometric Characterization of Murine B Cell Development
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Flow Cytometric Characterization of Murine B Cell Development

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Molecular- and flow cytometry-based diagnosis of primary immunodeficiency disorders

Joao B Oliveira1, Thomas A Fleisher

  • 1Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA. oliveirajb@cc.nih.gov

Current Allergy and Asthma Reports
|August 5, 2010
PubMed

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...