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Related Concept Videos

Molecular Models02:00

Molecular Models

Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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Protein Complexes with Interchangeable Parts01:57

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Articles linked to this work by shared authors, journal, and citation graph.

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Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs.

Ukrainian biochemical journal·2017
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[Insulin determination in pancreas of guinea pigs with metabolic syndrome].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2014
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[Stress-induced changes in the content of cytochrome P450 2E1 in the liver of mice with chronic psychoemotional overexertion].

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[Optimization of animal model for investigation of pathogenesis of type 2 diabetes].

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[Influence of long-term combined gamma-radiation and ethanol on cytochrome P450 2E1 expression in the mice liver].

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Related Experiment Video

Updated: Jun 10, 2026

Modeling an Enzyme Active Site using Molecular Visualization Freeware
14:37

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

[Computer modeling of the human cytochrome P-450 2E1 complex formation].

V O Kitam, M O Chashchyn

    Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
    |August 6, 2010
    PubMed
    Summary

    Computer models of human CYP2E1 spatial structures aid in understanding enzyme interactions. A reduced protein pocket volume may inhibit enzyme activity by blocking substrate access.

    Area of Science:

    • Biochemistry
    • Computational Biology
    • Enzymology

    Background:

    • Human CYP2E1 is a crucial enzyme involved in drug metabolism and detoxification.
    • Understanding its spatial structure is key to comprehending its function and interactions.
    • Computational methods offer a powerful tool for structural analysis and prediction.

    Purpose of the Study:

    • To compare experimentally determined and computationally derived spatial structures of human CYP2E1.
    • To validate a computational model for studying enzyme-substrate interactions.
    • To investigate potential mechanisms of enzyme inactivation.

    Main Methods:

    • Comparison of spatial structures using parameters like total energy, protein pocket volume, and molecular volume.
    • Analysis of spatial geometry.

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    Last Updated: Jun 10, 2026

    Modeling an Enzyme Active Site using Molecular Visualization Freeware
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    Published on: December 25, 2021

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    09:30

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    Published on: July 19, 2024

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  • Computational modeling and optimization of the enzyme structure.

    • Main Results:

    • The computationally derived and optimized model of human CYP2E1 closely matches experimental structures.
    • The model is suitable for studying the interaction mechanisms between the enzyme's active site and substrates/inhibitors.
    • A hypothesis was proposed regarding reduced protein pocket volume as a mechanism for enzyme inactivation.

    Conclusions:

    • Validated computational models can accurately represent enzyme spatial structures.
    • These models are valuable tools for elucidating enzyme mechanisms.
    • Reduced protein pocket volume is a potential mechanism for CYP2E1 inactivation, impacting substrate accessibility.